Specific Rotation Of Sucrose
The normalweight was then calculatedat first from theinverse ratioofspecific rotations of sucrose and dextrose.
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- Sucrose is wiidespread in seeds, leaves, fruits, flowers and roots of plants, where it functions as an energy store for metabolism and as a carbon source for biosynthesis. The annual world production of sucrose is in excess of 90 million tons mainly from the juice of sugar cane (20%) and sugar beet (17%).
- Glucose is combined into glucosides, disaccharides, oligosaccharides, the polysaccharides (cellulose and starch), and glycogen. Glucose is a mixture of α- and β-anomers, primarily the α-anomer. The optical rotation of the α-anomer is +112.2° (c = 10% in water, 20 °C) and the β-anomer is +18.7° (c = 10% in water, 20 °C).
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Standard solutions (5,10,25,50 g/100 mL) were analysed polarimetry and a calibration curve created, which was then used to determine the sucrose concentration of an unknown. (observed rotation 5.33)'Calculate specific rotation of sucrose from the slope of the graph'.I calculated the slope of the graph:difference in y values = 45difference in x values = 28y/x = 1.60So now I need to use the equation a/1 x cI am assuming its 1.6/1 x c.My question being which concentration do I use? An average of all the concentrations? We must also calculate concentration of the unknown using specific rotation, which Im unsure how to do. Im sure the answers to these questions will be very simple.Cheers. You haven't really described your thought process properly, so I will make an attempt at understanding what you mean:On your graph, I assume that X values are the concentrations (g/100mL) and the Y values are the observed rotation?y/x = 1.60So this is the slope of your curve. Thus, the equation for slope is Y = 1.60X.
Specific Rotation Of Sucrose Solution
From this equation you should be able to determine the concentration at any observed rotation.So now I need to use the equation a/1 x cThis is not an equation, as I do not see an equal sign. Write out the whole equation. What is the significance of this equation? Is it the absorbance equation? If so, the slope of your line is not what needs to be inputted here. It is the formula used to calculate the specific rotation.And you are correct in assuming the axis labels, sorry I didn't specify.where a = the obeserved rotation in circular degrees, this is where I'm confused, because we took readings for 5, 10, 25, 50 g/100 mL solutions of sucrose as well as an unknown. So do I plug the unknown in here?
What Is The Specific Rotation Of Sucrose
If so, why do I need the slope of the graph?1 = 1cm the pathlength and c = concentration, also confused as to which value I put in here, since there are five solutions.I'm just not sure whether I take an average, or I have to use the equation 5 times over, and THEN take an average, or what. Thanks for your awesome reply.
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